Silent Mutator is a silent mutagenesis scanning online tool. With Silent Mutator, you can easily introduce new restriction sites without changing the protein sequence.
Introduce restriction sites without changing amino acid sequence.
Gene synthesis design tool.
Synthetic biology online tool.
The sequence is:
Plain text, fasta or GenBank formats.
To display the cutting pattern and positions, double-click the enzyme name.
To display the cutting pattern and positions, double-click the enzyme name. To apply changes made in the selection, press 'Enter' key or click the 'Apply' button.
|B||C, G, T||V|
|D||A, G, T||H|
|H||A, C, T||D|
|V||A, C, G||B|
|N||A, C, G, T||N|
|N│G A T C N N C T A G│N||BamHI, BglII|
|N│A A T T N N T T A A│N||EcoRI, MfeI (MunI)|
|N│T C G A N N A G C T│N||SalI, XhoI|
|N│C T A G N N G A T C│N||AvrII (XmaJI), NheI, SpeI (BcuI), XbaI|
|N T G C A│N N│A C G T N||NsiI (Mph1103I), PstI|
|N│G T A C N N C A T G│N||Acc65I (Asp718I), BsiWI (Pfl23II), BsrGI (Bsp1407I)|
|N│C C G G N N G G C C│N||AgeI (BshTI), BspEI (Kpn2I), XmaI (Cfr9I)|
|N│G G C C N N C C G G│N||EagI (Eco52I), PspOMI (Bsp120I), NotI|
|N│C G C G N N G C G C│N||AscI (SgsI), BssHII (PauI), MluI|
|N N│C G N N N N G C│N N||AclI (Psp1406I), BstBI (Bsp119I), ClaI (Bsu15I), NarI|
|N N│T A N N N N A T│N N||AseI (VspI), NdeI|
|N N A T│N N N N│T A N N||PacI, PvuI|
|blunt ends||AfeI (Eco47III), BsaBI (BseJI), DraI, EcoRV (Eco32I), Ecl136II, FspI (NsbI), HpaI (KspAI), MscI (MlsI), NaeI (PdiI), NruI (Bsp68I), PmeI (MssI), PmlI (Eco72I), PvuII, ScaI, SfoI (EheI), SmaI, SnaBI (Eco105I), SrfI, SspI, StuI (Eco147I), SwaI (SmiI), XmnI (PdmI), ZraI|
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