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Molbiotools is a collection of free online apps:

DNA Sequence Tools
Text and Data Tools
Lab Calculators
Math Calculators

WebDSV has evolved!

The DNA sequence editor extended with new tools for restriction analysis, automated PCR primer design, restriction cloning and Golden Gate assembly is now a part of:

Sapio Sciences Electronic Lab Notebook

It has remained a free online app (for academics), you only need to sign up.

Screenshot Gallery

Plasmid editor
Search tool
Restriction analysis
Discerning cleavage suggestions
Restriction cloning wizard
Vector modification wizard