WebDSV is an online DNA sequence editor and map drawing program. It provides basic analysis of DNA sequences (restriction sites, GC-content).
A user can mark sequence features and visualize them along the sequence and as a feature map.
WebDSV can be used to perform plasmid cloning in silico, design PCR primers, or to plan a gene synthesis.
WebDSV is useful for molecular cloning and synthetic biology.
In silico gene editing tool.
WebDSV helps to visualize GenBank files. It is an online program to draw a map of features from a GenBank file
online DNA software, online molecular biology applications
WebDSV is a general-purpose molecular biology freeware, a DNA sequence manipulation suite, free plasmid cloning software, vector design application, DNA cloning program.
WebDSV is an online plasmid editor software, app to draw a plasmid map. It is a successor of Molecular Cloning Help.
WebDSV is a gene design software.
online app to design molecular cloning experiments, molecular cloning program, molecular cloning design
WebDSV is a DNA sequence viewer and editor. It is an online program that can open and edit GenBank files.
WebDSV is a plasmid design program.
WebDSV is an online tool to translate a DNA sequence to protein. It can also translate the DNA sequence in reverse orientation.
DNA translation, DNA translator, free DNA tools
online DNA to protein translation tool
online protein translation tool, nucleotide-protein translation
DNA sequence reverse complement tool
transcription and translation sequence tool, translate tool
It is a solution to plasmid mapping practice problems, an online vector editor. It facilitates DNA cloning design.
polylinker or multiple cloning sites region highlighting
restriction mapping of plasmid DNA
design PCR primers with restriction sites
design DNA cloning online
WebDSV is molecular biology software that allows users to plan, visualize, and document molecular biology procedures.
WebDSV contains a standard genetic code table.
WebDSV contains a table of compatible cohesive ends.
|Locus (or name):|
Copy other genetic codes from the Genetic Codes NCBI webpage.
|B||C, G, T||V|
|D||A, G, T||H|
|H||A, C, T||D|
|V||A, C, G||B|
|N||A, C, G, T||N|
|N│G A T C N N C T A G│N||BamHI, BglII|
|N│A A T T N N T T A A│N||EcoRI, MfeI (MunI)|
|N│T C G A N N A G C T│N||SalI, XhoI|
|N│C T A G N N G A T C│N||AvrII (XmaJI), NheI, SpeI (BcuI), XbaI|
|N T G C A│N N│A C G T N||NsiI (Mph1103I), PstI|
|N│G T A C N N C A T G│N||Acc65I (Asp718I), BsiWI (Pfl23II), BsrGI (Bsp1407I)|
|N│C C G G N N G G C C│N||AgeI (BshTI), BspEI (Kpn2I), XmaI (Cfr9I)|
|N│G G C C N N C C G G│N||EagI (Eco52I), PspOMI (Bsp120I), NotI|
|N│C G C G N N G C G C│N||AscI (SgsI), BssHII (PauI), MluI|
|N N│C G N N N N G C│N N||AclI (Psp1406I), BstBI (Bsp119I), ClaI (Bsu15I), NarI|
|N N│T A N N N N A T│N N||AseI (VspI), NdeI|
|N N A T│N N N N│T A N N||PacI, PvuI|
|blunt ends||AfeI (Eco47III), BsaBI (BseJI), DraI, EcoRV (Eco32I), Ecl136II, FspI (NsbI), HpaI (KspAI), MscI (MlsI), NaeI (PdiI), NruI (Bsp68I), PmeI (MssI), PmlI (Eco72I), PvuII, ScaI, SfoI (EheI), SmaI, SnaBI (Eco105I), SrfI, SspI, StuI (Eco147I), SwaI (SmiI), XmnI (PdmI), ZraI|
WebDSV calculates the selected sequence length, GC-content, and predicted melting temperature in real time when the user is selecting a sequence in the Editor Field. The melting temperature prediction is limited to length interval 10 - 120 bp and is based on the nearest neighbor method with parameters described in SantaLucia 1998 (see the WebDSV manual for more details and a full reference).
Click the GC-content value to see a GC-content plot.
To display the cutting pattern and positions, doubleclick the enzyme name. To apply changes made in the selection, press 'Enter' key or click the Apply button.
The restriction enzyme selection can be changed either manually with checkboxes located in the last column of the table or by loading the restriction enzyme list from a file with the Load List button. The file should be a plain text file containing one restriction enzyme name per line. You can write such file in MS Notepad or similar program or by saving the current selection with the Save List button. The Reset button will restore the default selection list. The Set Permanent button will store the present selection in the browser memory. The success of this operation depends on the particular web browser and its settings. If successful, the selection will be preserved in all new browser tabs or windows in the course of the present session. It, however, might not be available in future sessions, so saving the selection as a file is highly recommended.